Microalgae Analytical Methods

There are components of microalgae, in particular their polysaccharides and lipids, that are poorly characterized and utilized.   Analytical techniques have been developed to examine lipid, starch and cell wall carbohydrates. These methods have enabled discoveries on the impact of cultivation methods on flocculation of algae, cell wall lysis, and quality of accumulated products for biofuel production.

 

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Publications

Cheng Y-S, Labavitch J, VanderGheynst J (2011) High-throughput analysis of hexosamine using a colorimetric method Analytical Biochemistry 408: 160-162.

Cheng Y-S, Labavitch J, VanderGheynst JS (2011) The impact of cell wall carbohydrate composition on the chitosan flocculation of Chlorella. Process Biochemistry 46: 1927-1933.

Cheng Y-S, Labavitch J, VanderGheynst JS (2015) Elevated CO2 concentration impacts cell wall polysaccharide composition of green microalgae of the Genus Chlorella Letters in Applied Microbiology 60: 1-7.

Cheng Y-S, Labavitch J, VanderGheynst JS (2015) Organic and inorganic nitrogen impact Chlorella variabilis productivity and host quality for viral production and cell lysis. Applied Biochemistry and Biotechnology 176: 467-479.

Cheng Y-S, Zheng Y, Labavitch JM, VanderGheynst JS (2013) Virus infection of Chlorella variabilis and enzymatic saccharification of algal biomass for bioethanol production. Bioresource Technology 137: 326-331.

Cheng Y-S, Zheng Y, VanderGheynst JS (2010) Rapid quantitative analysis of lipid using a colorimetric method in a microplate format. Lipids 46: 95-103.

Higgins BT, Dunwoody A, Labavitch JM, VanderGheynst JS (2014) Microplate assay for quantitation of neutral lipids in extracts from microalgae. Analytical Biochemistry 465: 81-89.

Tanadul O-u-m, VanderGheynst JS, Beckles DM, Powell ALT, Labavitch JM (2014) The impact of elevated CO2 concentration on the quality of algal starch as a potential biofuel feedstock. Biotechnology and Bioengineering 111: 1323-1331.

Kind T, Meissen JK, Yang D, Nocito F, Vaniya A, et al. (2012) Qualitative analysis of algal secretions with multiple mass spectrometric platforms. Journal of Chromatography A 1244: 139-147.

Tsugawa H, Cajka T, Kind T, Ma Y, Higgins B, et al. (2015) MS-DIAL: Data independent MS/MS deconvolution for comprehensive metabolome analysis. Nature Methods 12: 523-526.

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